• Nitrogen fixation requires a strong reducing agent and energy in the form of ATP.
  • Nitrogen¬† is accomplished with the help of nitrogenfixing microbes, mainly Rhizobium.
  • Most of the processes take place in anaerobic environment.
  • The energy, ATP, required is provided by the respiration of the host cells.
  • Ammonia produced following N2 fixation is incorporated into amino acids as the amino group.

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